Active Recombinant Human BMP2 Protein (Gln283-Arg396), C-His tagged, Animal-free, Carrier-free

• Cat.No.: BMP2-277H
• Product Overview: Recombinant Human BMP2 Protein (Gln283-Arg396) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Bone Morphogenetic Protein-2 (BMP-2) is an extracellular multifunctional signaling cytokine that is also a member of the TGF-β family. BMP-2 can bind with the TGF-β receptor to active SMAD protein signal transduction. Moreover, it engages in the path of signals such as Wnt and Notch. BMP-2 plays an essential role in the growth of hard bones and cartilage and involved in the differentiation of osteoblasts.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 1.0 μg/mL.
• Molecular Mass: The protein has a calculated MW of 13.76 kDa.
  The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Gln283-Arg396
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: BMP2 bone morphogenetic protein 2 [ Homo sapiens (human) ]
• Official Symbol: BMP2
• Synonyms: BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A; bone morphogenetic protein 2A; BDA2
• Gene ID: 650
• mRNA Refseq: NM_001200
• Protein Refseq: NP_001191
• MIM: 112261
• UniProt ID: P12643