Active Recombinant Human BMP4 Protein (Lys303-Arg408), GMP Grade, Animal-Free

• Cat.No.: BMP4-35HG
• Product Overview: GMP recombinant human BMP-4 protein (Lys303-Arg408) without tag was expressed in E. coli, produced in an Animal-Free laboratory and manufactured and tested under cGMP guidelines.

Specification

• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Mutations in this gene are associated with orofacial cleft and microphthalmia in human patients. The encoded protein may also be involved in the pathology of multiple cardiovascular diseases and human cancers.
• Source: E. coli
• Species: Human
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 3-21 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 12 kDa
  SDS-PAGE: 8-9 kDa, reducing conditions
• Protein Length: Lys303-Arg408
• N-terminal Sequence Analysis: Identity was confirmed by mass spectrometry
• Endotoxin: <0.10 EU/ μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -65 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Reconstitution: Reconstitute at 50-200 μg/mL in sterile 4 mM HCl.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: BMP4 bone morphogenetic protein 4 [ Homo sapiens ]
• Official Symbol: BMP4
• Synonyms: BMP4; bone morphogenetic protein 4; BMP2B; BMP-4; BMP-2B; bone morphogenetic protein 2B; ZYME; OFC11; BMP2B1; MCOPS6
• Gene ID: 652
• mRNA Refseq: NM_001202
• Protein Refseq: NP_001193
• MIM: 112262
• UniProt ID: P12644