| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
16-688 aa |
| Description : |
This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency. |
| Form : |
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant. |
| Bio-activity : |
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5, 5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >14000 pmol/min/μg. |
| Molecular Mass : |
The protein has a calculated MW of 76.8 kDa. The protein migrates as 27 kDa, 30 kDa, 40 kDa, 53-55 kDa and 90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Endotoxin : |
Less than 1.0 EU/μg by the LAL method/rFC method. |
| Purity : |
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS. |
| Storage : |
This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
