Active Recombinant Human C1S, His-tagged

• Cat.No.: C1S-120H
• Product Overview: Recombinant Human C1S (Met1-Asp688) fused with 6­His tag at C­terminal, was expressed in Mouse NSO Cells.

Specification

• Description: This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency.
• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Glu16 (A chain) & Ile438 (B chain)
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >7,000 pmol/min/µg.
• Molecular Mass: 47 kDa (A chain) & 28 kDa (B chain)
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: C1S complement component 1, s subcomponent [ Homo sapiens(human) ]
• Official Symbol: C1S
• Synonyms: C1S; complement component 1, s subcomponent; complement C1s subcomponent; C1 esterase; basic proline-rich peptide IB-1; complement component 1 subcomponent s; EC 3.4.21.42
• Gene ID: 716
• mRNA Refseq: NM_001734
• Protein Refseq: NP_001725
• MIM: 120580
• UniProt ID: P09871
• Chromosome Location: 12p13
• Pathway: Classical antibody-mediated complement activation; Complement Activation; Creation of C4 and C2 activators
• Function: calcium ion binding; identical protein binding; serine-type endopeptidase activity