Recombinant Human C1S 293 Cell Lysate

• Cat.No.: C1S-8131HCL

Specification

• Description: Antigen standard for complement component 1, s subcomponent (C1S), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: C1S complement component 1, s subcomponent [ Homo sapiens ]
• Official Symbol: C1S
• Synonyms: C1S; complement component 1, s subcomponent; complement C1s subcomponent; C1 esterase; basic proline-rich peptide IB-1; complement component 1 subcomponent s; FLJ44757
• Gene ID: 716
• mRNA Refseq: NM_001734
• Protein Refseq: NP_001725
• MIM: 120580
• UniProt ID: P09871
• Chromosome Location: 12p13
• Pathway: Classical antibody-mediated complement activation, organism-specific biosystem; Complement Activation, Classical Pathway, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Creation of C4 and C2 activators, organism-specific biosystem
• Function: calcium ion binding; peptidase activity; serine-type endopeptidase activity