Active Recombinant Human CA10 Protein (22-328aa), C-His-tagged

• Cat.No.: CA10-16H
• Product Overview: Recombinant human Carbonic Anhydrase X/CA10 (22-328aa), fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Description: Carbonic anhydrase X, also known as CA10, belongs to the CA family of zinc metalloenzymes. It is catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. Also an acatalytic member of the alpha-carbonic anhydrase subgroup, and it is thought to play a role in the central nervous system, especially in brain development.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: Specific activity is > 150 pmol/min/μg, and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 8.0 at 37 centigrade.
• Molecular Mass: 36.3 kDa (317 aa)
• Protein length: 22-328 aa
• AA Sequence: QQNSPKIHEGWWAYKEVVQGSFVPV PSFWGLVNSAWNLCSVGKRQSPVNI ETSHMIFDPFLTPLRINTGGRKVSG TMYNTGRHVSLRLDKEHLVNISGGP MTYSHRLEEIRLHFGSEDSQGSEHL LNGQAFSGEVQLIHYNHELYTNVTE AAKSPNGLVVVSIFIKVSDSSNPFL NRMLNRDTITRITYKNDAYLLQGLN IEELYPETSSFITYDGSMTIPPCYE TASWIIMNKPVYITRMQMHSLRLLS QNQPSQIFLSMSDNFRPVQPLNNRC IRTNINFSLQGKDCPNNRAQKLQYR VNEWLLK
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Absorbance at 280 nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: CA10 carbonic anhydrase X [ Homo sapiens (human) ]
• Official Symbol: CA10
• Synonyms: CA10; carbonic anhydrase X; carbonic anhydrase-related protein 10; CA RPX; CARPX; HUCEP 15; CARP X; CA-RP X; cerebral protein 15; cerebral protein-15; carbonic anhydrase-related protein X; CA-RPX; HUCEP-15
• Gene ID: 56934
• mRNA Refseq: NM_020178
• Protein Refseq: NP_064563
• MIM: 604642
• UniProt ID: Q9NS85

Reviews

08/26/2022

Accurate protein characterization, facilitates data interpretation.

08/05/2021

Dependable quantification, ensures data accuracy.

11/03/2020

Expert support, indispensable for our protein experiments.

Q&As

What is the tissue-specific expression pattern of CA10 protein?

CA10 protein is primarily expressed in the kidneys and brain.

What is the role of CA10 protein in intracellular pH regulation?

CA10 protein contributes to intracellular pH regulation.

How does CA10 protein's enzymatic activity compare to other carbonic anhydrases?

CA10 protein's enzymatic activity varies compared to other carbonic anhydrases, and further research is needed to identify its specific substrates and catalytic mechanisms.

Can genetic models be developed to study CA10 protein's cellular roles?

Yes, genetic models like knockouts can be developed to study CA10 protein's cellular functions and associated phenotypic changes.

What are the primary tissues expressing CA10 protein?

CA10 protein is primarily expressed in the kidneys and brain.

What post-translational modifications regulate CA10 protein's activity and stability?

The specific post-translational modifications governing CA10 protein's activity and stability are under investigation.

Are there known disease associations with CA10 protein?

There are currently no widely reported disease associations or implications related to CA10 protein.