Recombinant Mouse CA10 cell lysate

• Cat.No.: CA10-1810MCL
• Product Overview: Mouse Ca10 / Car10 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: Car10 carbonic anhydrase 10 [ Mus musculus ]
• Official Symbol: Car10
• Synonyms: Ca10; BB085816; 2700029L05Rik; carbonic anhydrase-related protein 10; CARP X; CA-RP X; carbonic anhydrase-related protein X
• Gene ID: 72605
• mRNA Refseq: NM_028296
• Protein Refseq: NP_082572
• UniProt ID: P61215
• Chromosome Location: 11; 11 C
• Function: molecular_function

Reviews

08/26/2022

Accurate protein characterization, facilitates data interpretation.

08/05/2021

Dependable quantification, ensures data accuracy.

11/03/2020

Expert support, indispensable for our protein experiments.

Q&As

What is the tissue-specific expression pattern of CA10 protein?

CA10 protein is primarily expressed in the kidneys and brain.

What is the role of CA10 protein in intracellular pH regulation?

CA10 protein contributes to intracellular pH regulation.

How does CA10 protein's enzymatic activity compare to other carbonic anhydrases?

CA10 protein's enzymatic activity varies compared to other carbonic anhydrases, and further research is needed to identify its specific substrates and catalytic mechanisms.

Can genetic models be developed to study CA10 protein's cellular roles?

Yes, genetic models like knockouts can be developed to study CA10 protein's cellular functions and associated phenotypic changes.

What are the primary tissues expressing CA10 protein?

CA10 protein is primarily expressed in the kidneys and brain.

What post-translational modifications regulate CA10 protein's activity and stability?

The specific post-translational modifications governing CA10 protein's activity and stability are under investigation.

Are there known disease associations with CA10 protein?

There are currently no widely reported disease associations or implications related to CA10 protein.