Recombinant Human CA10 cell lysate

Cat.No. : CA10-2485HCL
Product Overview : Human CA10 / Carbonic anhydrase X derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CA10 carbonic anhydrase X [ Homo sapiens ]
Official Symbol : CA10
Synonyms : CA10; carbonic anhydrase X; carbonic anhydrase-related protein 10; CA RPX; CARPX; HUCEP 15; CARP X; CA-RP X; cerebral protein 15; cerebral protein-15; carbonic anhydrase-related protein X; CA-RPX; HUCEP-15;
Gene ID : 56934
mRNA Refseq : NM_001082533
Protein Refseq : NP_001076002
MIM : 604642
UniProt ID : Q9NS85
Chromosome Location : 17q21
Function : molecular_function;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What is the tissue-specific expression pattern of CA10 protein? 12/18/2022

CA10 protein is primarily expressed in the kidneys and brain.

What is the role of CA10 protein in intracellular pH regulation? 04/07/2021

CA10 protein contributes to intracellular pH regulation.

How does CA10 protein's enzymatic activity compare to other carbonic anhydrases? 03/29/2021

CA10 protein's enzymatic activity varies compared to other carbonic anhydrases, and further research is needed to identify its specific substrates and catalytic mechanisms.

Can genetic models be developed to study CA10 protein's cellular roles? 03/26/2018

Yes, genetic models like knockouts can be developed to study CA10 protein's cellular functions and associated phenotypic changes.

What are the primary tissues expressing CA10 protein? 12/25/2017

CA10 protein is primarily expressed in the kidneys and brain.

What post-translational modifications regulate CA10 protein's activity and stability? 09/04/2017

The specific post-translational modifications governing CA10 protein's activity and stability are under investigation.

Are there known disease associations with CA10 protein? 07/07/2016

There are currently no widely reported disease associations or implications related to CA10 protein.

Customer Reviews (3)

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Reviews
08/26/2022

    Accurate protein characterization, facilitates data interpretation.

    08/05/2021

      Dependable quantification, ensures data accuracy.

      11/03/2020

        Expert support, indispensable for our protein experiments.

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