| Description : |
WISP-1, also known as Wnt-1-inducible-signaling pathway protein 1, CCN4 and Wnt-1-induced secreted protein, is a cysteine-rich heparin-binding Glycoprotein belonging to the CCN protein family. It is expressed in many internal organs, such as the lung, kidney and spleen. WISP-1 binds to BMP-2 and enhances mesenchymal cell proliferation and osteoblastic differentiation., WISP-1 has also been reported to attenuate p53-mediated apoptosis and inhibit TNF-induced cell death, suggesting it may play a role intumorigenesis. |
| Source : |
CHO |
| Species : |
Human |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50< 0.5 μg/mL, measured in a bioassay using ATDC5 cells in the presence of 50 ng/mL human BMP-2. |
| Molecular Mass : |
44-46 kDa, observed by reducing SDS-PAGE. |
| AA Sequence : |
TALSPAPTTMDFTPAPLEDTSSRPQ FCKWPCECPPSPPRCPLGVSLITDG CECCKMCAQQLGDNCTEAAICDPHR GLYCDYSGDRPRYAIGVCAQVVGVG CVLDGVRYNNGQSFQPNCKYNCTCI DGAVGCTPLCLRVRPPRLWCPHPRR VSIPGHCCEQWVCEDDAKRPRKTAP RDTGAFDAVGEVEAWHRNCIAYTSP WSPCSTSCGLGVSTRISNVNAQCWP EQESRLCNLRPCDVDIHTLIKAGKK CLAVYQPEASMNFTLAGCISTRSYQ PKYCGVCMDNRCCIPYKSKTIDVSF QCPDGLGFSRQVLWINACFCNLSCR NPNDIFADLESYPDFSEIAN |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE. |
| Storage : |
Lyophilized recombinant human WISP-1 remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, human WISP-1 should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
