Recombinant Full Length Human CCN4 Protein, C-Flag-tagged

• Cat.No.: CCN4-1805HFL
• Product Overview: Recombinant Full Length Human CCN4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 38 kDa
• AA Sequence: MRWFLPWTLAAVTAAAASTVLATAL SPAPTTMDFTPAPLEDTSSRPQFCK WPCECPPSPPRCPLGVSLIT DGCECCKMCAQQLGDNCTEAAICDP HRGLYCDYSGDRPRYAIGVCAQVVG VGCVLDGVRYNNGQSFQPNC KYNCTCIDGAVGCTPLCLRVRPPRL WCPHPRRVSIPGHCCEQWVCEDDAK RPRKTAPRDTGAFDAVGEVE AWHRNCIAYTSPWSPCSTSCGLGVS TRISNVNAQCWPEQESRLCNLRPCD VDIHTLIKAGKKCLAVYQPE ASMNFTLAGCISTRSYQPKYCGVCM DNRCCIPYKSKTIDVSFQCPDGLGF SRQVLWINACFCNLSCRNPN DIFADLESYPDFSEIAN TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - Wnt Signaling pathway

Gene Information

• Gene Name: CCN4 cellular communication network factor 4 [ Homo sapiens (human) ]
• Official Symbol: CCN4
• Synonyms: WISP1; WISP1c; WISP1i; WISP1tc; WISP1-OT1; WISP1-UT1
• Gene ID: 8840
• mRNA Refseq: NM_003882.4
• Protein Refseq: NP_003873.1
• MIM: 603398
• UniProt ID: O95388