Active Recombinant Human CD22 Protein (Asp 20 - Arg 687), His-tagged, FITC-Labeled
Cat.No. : | CD22-335H |
Product Overview : | Recombinant Human CD22 Protein, His-tagged, FITC-Labeled is expressed from human HEK293 cells. It contains AA Asp 20 - Arg 687 (Accession # P20273-1). It is the FITC labeled form of Human Siglec-2 (Cat. No. CD2-H52H8). Predicted N-terminus: Asp 20. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 77.0 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Conjugate (FITC): Excitation source: 488 nm spectral line, argon-ion laser; Excitation Wavelength: 488 nm; Emission Wavelength: 535 nm. The FITC to protein molar ratio is 1.5-3.5. |
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Species : | Human |
Source : | HEK293 |
Tag : | His |
ProteinLength : | Asp 20 - Arg 687 |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Anti-Human CD22 MAb at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Siglec-2 (Cat. No. SI2-HF2H6) with a linear range of 0.625-10 μg/mL (Ex.488 nm/Em.535 nm) (QC tested). |
Molecular Mass : | 77.0 kDa |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name | CD22 CD22 molecule [ Homo sapiens ] |
Official Symbol | CD22 |
Synonyms | CD22; CD22 molecule; CD22 antigen; B-cell receptor CD22; SIGLEC 2; SIGLEC2; BL-CAM; SIGLEC-2; FLJ22814; MGC130020; |
Gene ID | 933 |
mRNA Refseq | NM_001185099 |
Protein Refseq | NP_001172028 |
MIM | 107266 |
UniProt ID | P20273 |
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Not For Human Consumption!
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