Active Recombinant Human CD22 Protein (Asp 20 - Arg 687), His-tagged, FITC-Labeled

• Cat.No.: CD22-335H
• Product Overview: Recombinant Human CD22 Protein, His-tagged, FITC-Labeled is expressed from human HEK293 cells. It contains AA Asp 20 - Arg 687 (Accession # P20273-1). It is the FITC labeled form of Human Siglec-2 (Cat. No. CD2-H52H8). Predicted N-terminus: Asp 20. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 77.0 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Conjugate (FITC): Excitation source: 488 nm spectral line, argon-ion laser; Excitation Wavelength: 488 nm; Emission Wavelength: 535 nm. The FITC to protein molar ratio is 1.5-3.5.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Anti-Human CD22 MAb at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Siglec-2 (Cat. No. SI2-HF2H6) with a linear range of 0.625-10 μg/mL (Ex.488 nm/Em.535 nm) (QC tested).
• Molecular Mass: 77.0 kDa
• Protein length: Asp 20 - Arg 687
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD22 CD22 molecule [ Homo sapiens ]
• Official Symbol: CD22
• Synonyms: CD22; CD22 molecule; CD22 antigen; B-cell receptor CD22; SIGLEC 2; SIGLEC2; BL-CAM; SIGLEC-2; FLJ22814; MGC130020
• Gene ID: 933
• mRNA Refseq: NM_001185099
• Protein Refseq: NP_001172028
• MIM: 107266
• UniProt ID: P20273