Active Recombinant Human CD22 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD22-482HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human CD22 (Accession # CAA42006) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is typically 0.03 - 0.15 μg/mL.
• Molecular Mass: Recombinant Human CD22, Fc Chimera has a calculated MW of 101.9 kDa (monomer). In SDS-PAGE migrates as 122-127 kDa, reducing conditions.
• N-terminal Sequence Analysis: Asp 20
• Purity: > 95 % by SDS-PAGE under reducing conditions and visualized by silver stain.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.

Gene Information

• Gene Name: CD22 CD22 molecule [ Homo sapiens ]
• Official Symbol: CD22
• Synonyms: CD22; CD22 molecule; CD22 antigen; B-cell receptor CD22; sialic acid binding Ig like lectin 2; SIGLEC 2; SIGLEC2; BL-CAM; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2; SIGLEC-2; FLJ22814; MGC130020
• Gene ID: 933
• mRNA Refseq: NM_001185099
• Protein Refseq: NP_001172028
• MIM: 107266
• UniProt ID: P20273