Active Recombinant Human CD27 protein, Fc-tagged, APC-labeled

• Cat.No.: CD27-1079HA
• Product Overview: Recombinant Human CD27 protein, Fc-tagged was Conjugated with NH2-Reactive APC(Allophycocyanin).

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Thr 21 - Ile 192
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD27, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, Avitag,Fc Tag (active trimer) with a linear range of 0.001-0.02 μg/mL.
• Molecular Mass: The protein has a calculated MW of 45.9 kDa. The protein migrates as 53-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
  >85% as determined by SEC-HPLC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD27 CD27 molecule [ Homo sapiens ]
• Official Symbol: CD27
• Synonyms: CD27; CD27 molecule; TNFRSF7, tumor necrosis factor receptor superfamily, member 7; CD27 antigen; S152; Tp55; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; T14; TNFRSF7; MGC20393
• Gene ID: 939
• mRNA Refseq: NM_001242
• Protein Refseq: NP_001233
• MIM: 186711
• UniProt ID: P26842