Active Recombinant Human CD274 protein, hFc-tagged

• Cat.No.: CD274-2330H
• Product Overview: Active Recombinant Human CD274 protein(Met 1-Thr 239)(NP_054862.1) was expressed in Human Cells with C-terminal fused Fc region of human IgG1.

Specification

• Species: Human
• Source: Human Cells
• Tag: Fc
• Protein Length: 1-239 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human PD-1 at 10 μg/ml (100 μl/well) can bind recombinant human B7-H1 / PD-L1 / Fc chimera with a linear range of 0.02-0.4 μg/ml.
• Molecular Mass: Measured by its binding ability in a functional ELISA. Immobilized human PD-1 at 10 μg/ml (100 μl/well) can bind recombinant human B7-H1 / PD-L1 / Fc chimera with a linear range of 0.02-0.4 μg/ml.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD274 CD274 molecule [ Homo sapiens ]
• Official Symbol: CD274
• Synonyms: CD274; CD274 molecule; CD274 antigen, PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296
• Gene ID: 29126
• mRNA Refseq: NM_014143
• Protein Refseq: NP_054862
• MIM: 605402
• UniProt ID: Q9NZQ7