Active Recombinant Human CD274 protein, mFc-tagged
Cat.No. : | CD274-2185H |
Product Overview : | Recombinant Human CD274 protein(NP_054862.1)(Met1-Thr239), fused with mFc tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | mFc |
Form : | Lyophilized from 20 mM Tris, 150 mM NaCl, 10 % Glycerol, pH 8.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its binding ability in a functional ELISA. Immobilized recombinant Human PD1-His at 10 μg/ml (100 μl/well) can bind human PD-L1 with a linear range of 1.28-20 μg/ml. 2. Labeled biotin to PD-L1 Protein, Human, Recombinant (ECD, Fc Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of PD-L1 Protein, Human, Recombinant (ECD, Fc Tag), Biotinylated bound to Atezolizumab was 0.1 nM. |
Molecular Mass : | The recombinant human CD274 consists of 455 amino acids and predicts a molecular mass of 51.7 kDa. As a result of glycosylation, the human CD274 migrates as an approximately 66 kDa protein in SDS-PAGE under reducing conditions. |
Protein Length : | Met1-Thr239 |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | CD274 CD274 molecule [ Homo sapiens ] |
Official Symbol : | CD274 |
Synonyms : | CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296; |
Gene ID : | 29126 |
mRNA Refseq : | NM_014143 |
Protein Refseq : | NP_054862 |
MIM : | 605402 |
UniProt ID : | Q9NZQ7 |
Products Types
◆ Recombinant Protein | ||
CD274-1366C | Recombinant Canine CD274 protein(Met1-Arg236), hFc-tagged, Biotinylated | +Inquiry |
CD274-706C | Recombinant Cynomolgus monkey CD274 Protein | +Inquiry |
CD274-707C | Recombinant Cynomolgus monkey CD274 Protein, Fc-tagged | +Inquiry |
CD274-76H | Recombinant Human CD274 Protein (F19-H240), C-6×His tagged | +Inquiry |
CD274-708H | Recombinant Human CD274 Protein | +Inquiry |
◆ Lysates | ||
CD274-002RCL | Recombinant Rat CD274 cell lysate | +Inquiry |
CD274-914CCL | Recombinant Cynomolgus CD274 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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