Active Recombinant Human CD274 protein, mFc-tagged

• Cat.No.: CD274-2185H
• Product Overview: Recombinant Human CD274 protein(NP_054862.1)(Met1-Thr239), fused with mFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: mFc
• Protein Length: Met1-Thr239
• Form: Lyophilized from 20 mM Tris, 150 mM NaCl, 10 % Glycerol, pH 8.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized recombinant Human PD1-His at 10 μg/ml (100 μl/well) can bind human PD-L1 with a linear range of 1.28-20 μg/ml. 2. Labeled biotin to PD-L1 Protein, Human, Recombinant (ECD, Fc Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of PD-L1 Protein, Human, Recombinant (ECD, Fc Tag), Biotinylated bound to Atezolizumab was 0.1 nM.
• Molecular Mass: The recombinant human CD274 consists of 455 amino acids and predicts a molecular mass of 51.7 kDa. As a result of glycosylation, the human CD274 migrates as an approximately 66 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD274 CD274 molecule [ Homo sapiens ]
• Official Symbol: CD274
• Synonyms: CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296
• Gene ID: 29126
• mRNA Refseq: NM_014143
• Protein Refseq: NP_054862
• MIM: 605402
• UniProt ID: Q9NZQ7