Active Recombinant Human CD276 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD276-756HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human CD276 (Accession # NP_079516), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is typically 3-12 μg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human CD276, Fc Chimera has a calculated MW of 50 kDa (monomer). In SDS-PAGE migrates as 70-80 kDa, reducing conditions.
• N-terminal Sequence Analysis: Leu 29
• Purity: > 95 % by SDS-PAGE under reducing conditions and visualized by silver stain.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Concentration: 100 μg/mL
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Conjugation: Alexa Fluor 555

Gene Information

• Gene Name: CD276 CD276 molecule [ Homo sapiens ]
• Official Symbol: CD276
• Synonyms: CD276; CD276 molecule; CD276 antigen; B7 H3; B7H3; B7RP 2; B7 homolog 3; costimulatory molecule; B7-H3; B7RP-2; 4Ig-B7-H3
• Gene ID: 80381
• mRNA Refseq: NM_001024736
• Protein Refseq: NP_001019907
• MIM: 605715
• UniProt ID: Q5ZPR3