Active Recombinant Human CD38 Protein, MIgG2a Fc-tagged, low endotoxin

• Cat.No.: CD38-362H
• Product Overview: Recombinant Human CD38 Protein, MIgG2a Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300 (Accession # P28907-1). Predicted N-terminus: Val 43. This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 56.9 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Species: Human
• Source: HEK293
• Tag: mIgG2a
• Protein Length: Val 43 - Ile 300
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Recombinant Human CD38 Protein, MIgG2a Fc-tagged, low endotoxin at 5 μg/mL (100 μL/well) can bind Anti-CD38 MAb, Human IgG1 with a linear range of 0.4-2 ng/mL (QC tested).
• Molecular Mass: 56.9 kDa
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD38 CD38 molecule [ Homo sapiens ]
• Official Symbol: CD38
• Synonyms: CD38; CD38 molecule; CD38 antigen (p45); ADP-ribosyl cyclase 1; T10
• Gene ID: 952
• mRNA Refseq: NM_001775
• Protein Refseq: NP_001766
• MIM: 107270
• UniProt ID: P28907