Active Recombinant Human CD3E & CD3D protein, His-Avi-tagged, Biotinylated, Low Endotoxin

• Cat.No.: CD3E & CD3D-3463H
• Product Overview: Biotinylated Recombinant Human CD3E & CD3D protein(P07766(CD3E) & P04234(CD3D))(23-126 aa(CD3E) & 22-105 aa(CD3D)), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 23-126 aa(CD3E) & 22-105 aa(CD3D)
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human CD3E&CD3D, His Avi Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 0.11μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 17.9 kDa (CD3E) and 18.52 kDa (CD3D). Due to glycosylation, the protein migrates to 23-27 kDa and 32-40 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• UniProt ID: P07766
• Gene Name 2: CD3D CD3d molecule, delta (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol 2: CD3D
• Synonyms 2: CD3D; CD3d molecule, delta (CD3-TCR complex); CD3d antigen, delta polypeptide (TiT3 complex) , T3D; T-cell surface glycoprotein CD3 delta chain; CD3 delta; OKT3, delta chain; CD3 antigen, delta subunit; T-cell receptor T3 delta chain; CD3d antigen, delta polypeptide (TiT3 complex); T3D; CD3-DELTA
• Gene ID 2: 915
• mRNA Refseq 2: NM_000732
• Protein Refseq 2: NP_000723
• MIM 2: 186790
• UniProt ID 2: P04234