Active Recombinant Human CD40L protein, His-tagged

• Cat.No.: CD40L-326H
• Product Overview: Recombinant Human CD40L protein(NP_000065.1)(Glu108-Leu261), fused with His tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Glu108-Leu261
• Form: Lyophilized from sterile PBS, pH 7.4, 10% glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Human CD40 Ligand (His Tag) at 2 μg/mL (100 μL/well) can bind Human CD40 (ECD, hFc Tag), the EC50 of Human CD40 (ECD, hFc Tag) is 60-600 ng/mL.
• Molecular Mass: The recombinant human CD40L consists of 161 amino acids and has a calculated molecular mass of 17.7 kDa. It migrates as an 18 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.