Active Recombinant Human EFNB2 protein, Fc-tagged

• Cat.No.: EFNB2-1403H
• Product Overview: Recombinant Human EFNB2 protein(Ser25-Ala168), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5.
• Bio-activity: Immobilized Nipah virus Glycoprotein, His Tag at 5 μg/mL (100 μL/well) can bind Human Ephrin-B2 Protein, Fc tag with a linear range of 0.1-2 ng/mL.Immobilized Hendra virus Glycoprotein, His Tag at 5 μg/mL (100 μL/well) can bind Human Ephrin-B2 Protein, Fc tag with a linear range of 0.1-2 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 42.8 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ser25-Ala168
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EFNB2 ephrin-B2 [ Homo sapiens ]
• Official Symbol: EFNB2
• Synonyms: EFNB2; ephrin-B2; EPLG5; eph related receptor tyrosine kinase ligand 5; HTK ligand; Htk L; HTKL; LERK5; ligand of eph related kinase 5; MGC126226; MGC126227; MGC126228; LERK-5; ligand of eph-related kinase 5; eph-related receptor tyrosine kinase ligand 5; Htk-L
• Gene ID: 1948
• mRNA Refseq: NM_004093
• Protein Refseq: NP_004084
• MIM: 600527
• UniProt ID: P52799