Active Recombinant Human EGFR Protein, His-tagged

• Cat.No.: EGFR-1525H
• Product Overview: Active Recombinant Human EGFR Protein(Met 1-Lys 642)(NP_005219) was expressed in Insect Cells with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-642 aa
• Form: Lyophilized from sterile 20mM Tris, 50mM NaCl, 3mM DTT, 0.5mM TCEP, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human EGF at 10 μg/ml can bind human EGFR with a linear range of 3.2-400 ng/ml.
• Molecular Mass: The recombinant human EGFR consists of 626 amino acids and has a calculated molecular mass of 69.2 kDa. The recombinant protein migrates as an approximately 75 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog), ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533