Active Recombinant Human EGFR Protein (Leu 25 - Ser 645), His-tagged, low endotoxin
Cat.No. : | EGFR-338H |
Product Overview : | Recombinant Human EGFR Protein, His-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1). Predicted N-terminus: Leu 25. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.5kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Recombinant Human EGFR Protein, His-tagged, low endotoxin with a linear range of 0.8-6 ng/mL (QC tested). Immobilized Erbitux on CM5 Chip via anti-human Fc IgG, can bind Recombinant Human EGFR Protein, His-tagged, low endotoxin with an affinity constant of 0.492 nM as determined in SPR assay (Biacore T200) (Routinely tested). |
Molecular Mass : | 70.5kDa |
Protein length : | Leu 25 - Ser 645 |
Endotoxin : | Less than 0.01 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | EGFR epidermal growth factor receptor [ Homo sapiens ] |
Official Symbol : | EGFR |
Synonyms : | EGFR; epidermal growth factor receptor; ERBB1; ERBB; HER1; mENA; PIG61; |
Gene ID : | 1956 |
mRNA Refseq : | NM_005228 |
Protein Refseq : | NP_005219 |
MIM : | 131550 |
UniProt ID : | P00533 |
Products Types
◆ Recombinant Protein | ||
EGFR-1369H | Recombinant Human EGFR Protein, DDK-tagged | +Inquiry |
EGFR-072H | Recombinant Human EGFRviii Protein, DYKDDDDK/His-tagged | +Inquiry |
EGFR-077H | Recombinant Human EGFR Protein, DYKDDDDK-tagged | +Inquiry |
EGFR-211H | Recombinant Human EGFR Protein, His-tagged | +Inquiry |
EGFR-337H | Active Recombinant Human EGFRvIII Protein (Leu 25 - Ser 645), His-tagged | +Inquiry |
◆ Lysates | ||
EGFR-2733HCL | Recombinant Human EGFR cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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