Active Recombinant Human EGFR protein, lFc-tagged

• Cat.No.: EGFR-344H
• Product Overview: Recombinant Human EGFR protein(Leu 25 - Ser 645), fused with Llama IgG2b Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: lIgG2b
• Protein Length: 25-645 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human EGF, His Tag at 5 μg/mL (100 μL/well) can bind Human EGF R, Llama IgG2b Fc Tag with a linear range of 0.2-5 ng/mL.Immobilized Human EGF R, Llama IgG2b Fc Tag at 2 μg/mL (100 μL/well) can bind Cetuximab with a linear range of 0.3-5 ng/mL.Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
• Molecular Mass: This protein carries a llama IgG2b Fc tag at the C-terminus.The protein has a calculated MW of 96.4 kDa. The protein migrates as 115-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu25-Ser645
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533