Active Recombinant Human ENPP3 protein, His-Avi-tagged, Biotinylated

• Cat.No.: ENPP3-2205H
• Product Overview: Biotinylated Recombinant Human ENPP3 protein(Leu 48 - Ile 875), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Leu48-Ile875
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Measured by its ability to hydrolyze the Pyrophosphate (PPi) from the substrate adenosine-5’-triphosphate (ATP). The specific activity is > 100 pmol/min/μg.
• Molecular Mass: This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag.The protein has a calculated MW of 98.6 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: ENPP3 ectonucleotide pyrophosphatase/phosphodiesterase 3 [ Homo sapiens ]
• Official Symbol: ENPP3
• Synonyms: ENPP3; ectonucleotide pyrophosphatase/phosphodiesterase 3; PDNP3; ectonucleotide pyrophosphatase/phosphodiesterase family member 3; B10; CD203c; gp130RB13 6; PD IBETA; E-NPP 3; gp130RB13-6; phosphodiesterase I beta; phosphodiesterase-I beta; phosphodiesterase I/nucleotide pyrophosphatase 3; dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); NPP3; PD-IBETA
• Gene ID: 5169
• mRNA Refseq: NM_005021
• Protein Refseq: NP_005012
• MIM: 602182
• UniProt ID: O14638