Active Recombinant Canine ENPP3 protein, His-tagged, Low Endotoxin

• Cat.No.: ENPP3-1446C
• Product Overview: Recombinant Canine ENPP3 protein(A0A8I3N0F5)(46-874 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: His
• Protein Length: 46-874 aa
• Form: Supplied as 0.22μm filtered solution in 50mM Tris, 150mM NaCl (pH 7.5).
• Bio-activity: Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is > 8000 pmol/min/μg.
• Molecular Mass: The protein has a predicted MW of 96.19 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Concentration: 0.1-1.0 mg/mL