Active Recombinant Human ERAP2 protein, His-tagged

• Cat.No.: ERAP2-304H
• Product Overview: Active Recombinant Human ERAP2 protein(NP_071745.1) (Ala 56-Thr 960) was expressed in HEK293 with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 56-960 aa
• Form: Lyophilized from sterile 12.5mM Tris, 75mM NaCl, pH 7.5, 50% glycercol.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (Arg-AMC). The specific activity is >50 pmoles/min/μg.
• Molecular Mass: The recombinant human ERAP2 consists of 921 amino acids and predictes a molecular mass of 106 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 115-125 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • Chemoproteomics validates selective targeting of Plasmodium M1 alanyl aminopeptidase as a cross-species strategy to treat malaria (2023)
  • On-target, dual aminopeptidase inhibition provides cross-species antimalarial activity (2023)
  • Chemoproteomics validates selective targeting of Plasmodium M1 alanyl aminopeptidase as an antimalarial strategy (2024)

Gene Information

• Gene Name: ERAP2 endoplasmic reticulum aminopeptidase 2 [ Homo sapiens ]
• Official Symbol: ERAP2
• Synonyms: ERAP2; endoplasmic reticulum aminopeptidase 2; L RAP; leukocyte derived arginine aminopeptidase; LRAP; leukocyte-derived arginine aminopeptidase; L-RAP; FLJ23633; FLJ23701; FLJ23807
• Gene ID: 64167
• mRNA Refseq: NM_001130140
• Protein Refseq: NP_001123612
• MIM: 609497
• UniProt ID: Q6P179