Active Recombinant Human ERBB2 Protein, Fc/His-tagged, Alexa Fluor® 488 conjugated

• Cat.No.: ERBB2-13H
• Product Overview: Recombinant human Alexa Fluor® 488 conjugated Erb B2 (Thr23-Thr652) protein with human IgG1 (Pro100-Lys330) Fc tag and His tag at C-terminus was expressed in mouse myeloma cell line.

Specification

• Description: This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
• Source: Mouse myeloma cell line
• Species: Human
• Tag: Fc/His
• Form: Disulfide-linked homodimer
  Labeled with Alexa Fluor® 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515-545 nm
• Bio-activity: Measured by flow cytometry for its ability to bind anti-Human ErbB2/Her2 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human ErbB2/Her2 Fc Chimera Alexa Fluor® 488 that produces 50% of the binding response is 10.0-100 ng/mL.
• Molecular Mass: 97 kDa
• N-terminal Sequence Analysis: Thr23
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after opening.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: ERBB2 erb-b2 receptor tyrosine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: ERBB2
• Synonyms: ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; VSCN2; MLN 19; c-ERB2; c-ERB-2; HER-2/neu; receptor tyrosine-protein kinase erbB-2; c-erb B2/neu protein; herstatin; human epidermal growth factor receptor 2; metastatic lymph node gene 19 protein; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; proto-oncogene Neu; proto-oncogene c-ErbB-2; tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; EC 2.7.10.1
• Gene ID: 2064
• mRNA Refseq: NM_004448
• Protein Refseq: NP_004439
• MIM: 164870
• UniProt ID: P04626