Active Recombinant Human FAS, Fc-tagged, Biotinylated

• Cat.No.: FAS-603H
• Product Overview: The recombinant human Fas-Fc fusion is expressed as a 376 amino acid protein consisting of Gln26 - Asn173 region of Fas (UniProt accession #P25445) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions

Specification

• Source: Human cells
• Species: Human
• Tag: Fc
• Form: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule).
• Bio-activity: Binds to its ligand FasL and inhibits FasL-induced apoptosis in human acute T cell leukemia Jurkat cells.
• Molecular Mass: Calculated molecular mass (kDa): 42.2; Estimated by SDS-PAGE under reducing condition (kDa): 55-60
• Endotoxin: <0.1 eu per 1 μg of purified recombinant protein determined by the
• Purity: >95% judged by SDS-PAGE under reducing condition
• Storage: The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Avoid repeated freeze-thaw cycles.
• Protein length: Gln26-Asn173

Gene Information

• Gene Name: FAS Fas (TNF receptor superfamily, member 6) [ Homo sapiens ]
• Official Symbol: FAS
• Synonyms: FAS; Fas (TNF receptor superfamily, member 6); APT1, FAS1, TNFRSF6, tumor necrosis factor receptor superfamily, member 6; tumor necrosis factor receptor superfamily member 6; APO 1; CD95; Fas AMA; FAS 827dupA; CD95 antigen; FASLG receptor; apoptosis antigen 1; Delta Fas/APO-1/CD95; APO-1 cell surface antigen; apoptosis-mediating surface antigen FAS; tumor necrosis factor receptor superfamily, member 6; APT1; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• Gene ID: 355
• mRNA Refseq: NM_000043
• Protein Refseq: NP_000034
• MIM: 134637
• UniProt ID: P25445
• Chromosome Location: 10q24.1
• Pathway: Activation of Pro-Caspase 8, organism-specific biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Alzheimers disease, organism-specific biosystem
• Function: binding; identical protein binding; kinase binding; protein binding; receptor activity; receptor activity; signal transducer activity; transmembrane signaling receptor activity; tumor necrosis factor-activated receptor activity

Reviews

09/07/2022

When experimenting with FAS, the results are very reproducible.

07/29/2022

The stability of FAS products allows them to cope with different experimental conditions and modes of operation.

09/20/2021

FAS has high specific activity and can exert strong biological effect at low concentration.

Q&As

Does FAS protein play a role in cell signaling?

FAS protein plays an important role in cell signaling, it can activate downstream signaling pathways, such as death signaling pathway and survival signaling pathway, by binding to its ligand and transmitting signals.

Why apply knowledge of FAS proteins to clinical practice?

It can provide a reference for clinical practice and guide the diagnosis, treatment and prevention of diseases by detecting the expression level of FAS protein and understanding its mechanism of action.

How can diseases associated with FAS protein be prevented and treated?

FAS protein-related diseases can be prevented and treated by regulating the expression level of FAS protein and inhibiting its signaling pathway.

How can gene knockout or knockdown techniques be used to study the function of FAS proteins?

Gene knockout or knockdown techniques can be used to study the function and mechanism of action of FAS proteins. By knocking out or knocking down the FAS gene, changes in processes such as apoptosis and immune response can be observed to further understand its function and mechanism of action.

What is the role of FAS protein in apoptosis?

FAS protein can bind to its ligand, activate the death signaling pathway, and induce apoptosis.

How can FAS protein be used to predict disease prognosis?

The expression level and activity of FAS protein can be measured to predict the prognosis and development trend of certain diseases.