Active Recombinant Human FASLG Protein (Gln130-Leu281), N-His-SUMO tagged, Animal-free, Carrier-free

• Cat.No.: FASLG-166H
• Product Overview: Recombinant Human FASLG Protein (Gln130-Leu281) with N-His-SUMO tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Fas Ligand (FasL) is a 17.34 kDa tumor necrosis factor with 152 amino acid residues. FasL is expressed from lymphoid tissue and secreted to blood. Binding to its receptor, TNFRSF6/FAS, leads to induce apoptotic signal into cells. Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development.
• Source: E. coli
• Species: Human
• Tag: N-His-SUMO
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce apoptosis in Jurkat cells. The ED50 for this effect is < 1 ng/mL. The specific activity of recombinant human FasL is > 1 × 10⁶ IU/mg.
• Molecular Mass: The protein has a calculated MW of 29.51 kDa.
  The protein migrates as 30-35 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Gln130-Leu281
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: FASLG Fas ligand (TNF superfamily, member 6) [ Homo sapiens (human) ]
• Official Symbol: FASLG
• Synonyms: FASLG; Fas ligand (TNF superfamily, member 6); APT1LG1, TNFSF6, tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6; CD178; FasL; APTL; CD95 ligand; fas antigen ligand; apoptosis antigen ligand; apoptosis (APO-1) antigen ligand 1; tumor necrosis factor (ligand) superfamily, member 6; FASL; CD95L; CD95-L; TNFSF6; APT1LG1
• Gene ID: 356
• mRNA Refseq: NM_000639
• Protein Refseq: NP_000630
• MIM: 134638
• UniProt ID: P48023