Active Recombinant Human FGF18 Protein (174 aa)

• Cat.No.: FGF18-418F
• Product Overview: Recombinant human Fibroblast Growth Factor-18 (rhFGF-18) produced in E. coli is a single non-glycosylated polypeptide chain containing 174 amino acids. A fully biologically active molecule, rhFGF-18 has a molecular mass of 20.3 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.

Specification

• Description: Fibroblast Growth Factor-18 (FGF-18) is a heparin-binding growth factor that is a member of the FGF family. FGF-18 signals through FGFR 1c, 2c, 3c, and 4. FGF-18 plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. FGF-18 is required for normal ossification and bone development. It can also stimulate hepatic and intestinal proliferation.
• Source: E. coli
• Species: Human
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50 < 10 ng/mL, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 1.0 × 10^5units/mg.
• Molecular Mass: 20.3 kDa, observed by reducing SDS-PAGE.
• Protein Length: 174
• AA Sequence: MAEENVDFRIHVENQTRARDDVSRK QLRLYQLYSRTSGKHIQVLGRRISA RGEDGDKYAQLLVETDTFGSQVRIK GKETEFYLCMNRKGKLVGKPDGTSK ECVFIEKVLENNYTALMSAKYSGWY VGFTKKGRPRKGPKTRENQQDVHFM KRYPKGQPELQKPFKYTTVTKRSR
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% by SDS-PAGE and HPLC analyses.
• Storage: Lyophilized recombinant human Fibroblast Growth Factor-18 (rhFGF-18) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhFGF-18 should be stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Gene Information

• Gene Name: FGF18 fibroblast growth factor 18 [ Homo sapiens ]
• Official Symbol: FGF18
• Synonyms: FGF18; fibroblast growth factor 18; FGF 18; ZFGF5; FGF-18
• Gene ID: 8817
• mRNA Refseq: NM_003862
• Protein Refseq: NP_003853
• MIM: 603726
• UniProt ID: O76093

Reviews

01/09/2022

the FGF18 protein exhibits remarkable stability, enabling it to maintain its structural integrity and functionality throughout the experimental process.

05/30/2021

The FGF18 protein offers exceptional quality and purity, which is crucial for ensuring reliable and reproducible experimental outcomes.

12/08/2017

As a researcher utilizing the FGF18 protein, I am fortunate to benefit from its numerous advantages in trials.

Q&As

What are the potential side effects of FGF18 therapy?

While generally considered safe, potential side effects may include local reactions at the injection site. Comprehensive studies are ongoing to assess long-term safety.

Is FGF18 only applicable to bone-related conditions?

While its primary focus is on musculoskeletal conditions, FGF18's role in cellular growth and repair suggests potential applications in other medical fields.

How does FGF18 compare to other growth factors in terms of clinical applications?

Comparative studies are underway to evaluate the effectiveness and safety of FGF18 against other growth factors in various clinical scenarios.

Are there any ongoing clinical trials involving FGF18?

Yes, there are several clinical trials exploring the safety and efficacy of FGF18 in various musculoskeletal disorders and conditions.

How does FGF18 contribute to the treatment of osteoarthritis?

FGF18 has shown promise in promoting cartilage repair and regeneration, making it a potential candidate for the development of therapies for osteoarthritis.