Active Recombinant Human FGF7 Protein (181 aa), N-His-tagged

• Cat.No.: FGF7-346F
• Product Overview: Recombinant human Keratinocyte Growth Factor (rhKGF) with N-terminal His-tag produced in E. coli is a single non-glycosylated polypeptide chain containing 181 amino acids. A fully biologically active molecule, rhKGF has a molecular mass of 21.2 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.

Specification

• Description: Keratinocyte Growth Factor (KGF) is a highly specific epithelial mitogen produced by fibroblasts and mesenchymal stem cells. KGF belongs to the heparin binding Fibroblast Growth Factor (FGF) family, and is known as FGF-7. However, in contrast to the FGF-1, which binds to all known FGF receptors with high affinity, KGF only binds to a splice variant of an FGF receptor, FGFR2-IIIb. FGFR2-IIIb is produced by most of the epithelial cells, indicating that KGF plays roles as a paracrine mediator. KGF induces the differen-tiation and proliferation of various epithelial cells, including keratinocytes in the epidermis, hair follicles and sebaceous glands, and is responsible for the wound repairs of various tissues, including lung, bladder, and kidney.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50 < 10 ng/mL, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 1.0 × 10^5 units/mg.
• Molecular Mass: 21.2 kDa, observed by reducing SDS-PAGE.
• Protein length: 181
• AA Sequence: MNHKVHHHHHHMDDDDKMCNDMTPE QMATNVNCSSPERHTRSYDYMEGGD IRVRRLFCRTQWYLRIDKRGKVKGT QEMKNNYNIMEIRTVAVGIVAIKGV ESEFYLAMNKEGKLYAKKECNEDCN FKELILENHYNTYASAKWTHNGGEM FVALNQKGIPVRGKKTKKEQKTAHF LPMAILKERIEENGYT
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% by SDS-PAGE and HPLC analyses.
• Storage: Lyophilized recombinant human Keratinocyte Growth Factor (rhKGF) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhKGF should be stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Gene Information

• Gene Name: FGF7 fibroblast growth factor 7 [ Homo sapiens ]
• Official Symbol: FGF7
• Synonyms: FGF7; fibroblast growth factor 7; fibroblast growth factor 7 (keratinocyte growth factor); keratinocyte growth factor; KGF; FGF-7; heparin-binding growth factor 7; HBGF-7
• Gene ID: 2252
• mRNA Refseq: NM_002009
• Protein Refseq: NP_002000
• MIM: 148180
• UniProt ID: P21781

Reviews

03/21/2022

I highly recommend the FGF7 Protein to fellow researchers seeking a high-quality protein that not only meets their experimental requirements but is also accompanied by exceptional technical support.

05/01/2021

The FGF7 Protein's consistent stability and robust performance make it an indispensable tool for studying its multifaceted roles and mechanisms within vital biochemical pathways and cellular processes.

10/01/2017

What truly distinguishes the FGF7 Protein is the unparalleled technical support provided by the manufacturer.

Q&As

How does FGF7 aid in tissue repair?

FGF7 stimulates the proliferation and migration of epithelial cells, which are crucial for repairing damaged tissues.

Are there any side effects associated with FGF7 treatment?

Side effects are generally mild, such as local irritation at the site of application, but comprehensive studies are ongoing.

Can FGF7 be used in regenerative medicine?

Yes, FGF7 is being explored for its potential in regenerating tissues and promoting healing in various medical fields.

How is FGF7 administered in clinical settings?

FGF7 can be administered topically or via injection, depending on the specific condition being treated.

Is FGF7 used in dermatology?

Yes, FGF7 has been investigated for its potential in treating skin disorders, including conditions like psoriasis and atopic dermatitis.