Recombinant Human FGF7 293 Cell Lysate

• Cat.No.: FGF7-6236HCL

Specification

• Description: Antigen standard for fibroblast growth factor 7 (keratinocyte growth factor) (FGF7) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: FGF7 fibroblast growth factor 7 [ Homo sapiens ]
• Official Symbol: FGF7
• Synonyms: FGF7; fibroblast growth factor 7; fibroblast growth factor 7 (keratinocyte growth factor); keratinocyte growth factor; KGF; FGF-7; heparin-binding growth factor 7; HBGF-7
• Gene ID: 2252
• mRNA Refseq: NM_002009
• Protein Refseq: NP_002000
• MIM: 148180
• UniProt ID: P21781
• Chromosome Location: 15q21.2
• Pathway: Downstream signaling of activated FGFR, organism-specific biosystem; FGFR ligand binding and activation, organism-specific biosystem; FGFR2 ligand binding and activation, organism-specific biosystem; FGFR2b ligand binding and activation, organism-specific biosystem; FRS2-mediated cascade, organism-specific biosystem; Glypican 3 network, organism-specific biosystem; IRS-mediated signalling, organism-specific biosystem
• Function: chemoattractant activity; growth factor activity; heparin binding; type 2 fibroblast growth factor receptor binding

Reviews

03/21/2022

I highly recommend the FGF7 Protein to fellow researchers seeking a high-quality protein that not only meets their experimental requirements but is also accompanied by exceptional technical support.

05/01/2021

The FGF7 Protein's consistent stability and robust performance make it an indispensable tool for studying its multifaceted roles and mechanisms within vital biochemical pathways and cellular processes.

10/01/2017

What truly distinguishes the FGF7 Protein is the unparalleled technical support provided by the manufacturer.

Q&As

How does FGF7 aid in tissue repair?

FGF7 stimulates the proliferation and migration of epithelial cells, which are crucial for repairing damaged tissues.

Are there any side effects associated with FGF7 treatment?

Side effects are generally mild, such as local irritation at the site of application, but comprehensive studies are ongoing.

Can FGF7 be used in regenerative medicine?

Yes, FGF7 is being explored for its potential in regenerating tissues and promoting healing in various medical fields.

How is FGF7 administered in clinical settings?

FGF7 can be administered topically or via injection, depending on the specific condition being treated.

Is FGF7 used in dermatology?

Yes, FGF7 has been investigated for its potential in treating skin disorders, including conditions like psoriasis and atopic dermatitis.