Active Recombinant Human FLT3, Fc Chimera

• Cat.No.: FLT3-155H
• Product Overview: Recombinant Human FLT3 (Accession # AAA18947) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Predicted N Terminal: Asn27
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit Flt-3 Ligand-induced proliferation of BaF3 mouse pro-B cells transfected with Flt-3.The ED50 for this effect is typically 0.01 - 0.03 µg/mL in the presence of rhFlt-3 Ligand at 2 ng/mL.
• Molecular Mass: Recombinant Human FLT3, Fc Chimera has a calculated MW of 85.5 kDa (monomer). In SDS-PAGE migrates as 120 kDa, reducing conditions.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 50 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: FLT3 fms-related tyrosine kinase 3 [ Homo sapiens ]
• Official Symbol: FLT3
• Synonyms: FLT3; fms-related tyrosine kinase 3; receptor-type tyrosine-protein kinase FLT3; CD135; FLK2; STK1; STK-1; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; stem cell tyrosine kinase 1; growth factor receptor tyrosine kinase type III; FLK-2
• Gene ID: 2322
• mRNA Refseq: NM_004119
• Protein Refseq: NP_004110
• MIM: 136351
• UniProt ID: P36888
• Chromosome Location: 13q12