Active Recombinant Human FLT3LG Protein (Thr27-Ala181), C-His tagged, Animal-free, Carrier-free

Cat.No. : FLT3LG-110H
Product Overview : Recombinant Human FLT3LG Protein (Thr27-Ala181) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.
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Description : Fms-related tyrosine kinase-3 ligand (Flt-3 Ligand) is a protein which is encoded by the FLT3LG gene in human. As an important regulator of hematopoiesis, Flt-3 ligand has a tyrosine-protein kinase activity that stimulate the proliferation of hematopoietic progenitor cells of both lymphoid and myeloid origin. Flt-3 ligand can synergistically increase the proliferation of immature progenitors with other cytokines such as GM-CSF, G-CSF, IL-3, EPO, IL-11, IL-12, IL-6 or TPO. Flt-3 ligand is expressed by a variety of hematopoietic progenitor cells including immature hematopoietic and marrow stromal cells.
Source : E. coli
Species : Human
Tag : His
Form : Lyophilized
Bio-activity : Measure by its ability to induce proliferation in BaF3 cells transfected with mouse Flt-3. The ED50 for this effect is < 0.8 ng/mL. The specific activity of recombinant human Flt-3 Ligand is > 1.5 × 10⁶ IU/mg.
Molecular Mass : The protein has a calculated MW of 18.6 kDa.
The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis).
Protein Length : Thr27-Ala181
Endotoxin : < 0.1 EU/μg of the protein by the LAL method.
Purity : >95% as determined by SDS-PAGE analysis.
Storage : This product is stable after storage at:
-20 centigrade for 12 months in lyophilized state from date of receipt.
-20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Storage Buffer : Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
Reconstitution : It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping : The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.
Gene Name : FLT3LG fms-related tyrosine kinase 3 ligand [ Homo sapiens (human) ]
Official Symbol : FLT3LG
Synonyms : FLT3LG; fms-related tyrosine kinase 3 ligand; flt3 ligand; FL; FLT3L;
Gene ID : 2323
mRNA Refseq : NM_001204502
Protein Refseq : NP_001191431
MIM : 600007
UniProt ID : P49771

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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