Active Recombinant Human Frizzled Family Receptor 10, Fc-tagged

• Cat.No.: FZD10-581H
• Product Overview: RecombinantHuman FZD10 protein was expressed in Murine myeloma cell line with the Fcpart of IgG1 Pro100-Lys330 (C-terminus) and Human Frizzled 10Ile21Gly161 (N-terminus).

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Description: FZD10 is a 68 kDa sevenpass transmembrane glycoprotein in the Frizzled family of Wnt receptors. The 205amino acid N-terminal extracellular region of FZD10 contains a cysteine-rich domainthat comprises the Wnt binding domain and mediates receptor oligomerization.
• Form: Supplied as a 0.2 μmfiltered solution in PBS.
• N-terminal Sequence: Ile21
• Molecular Weight: 43 kDa
• Activity: Measured by itsbinding ability in a functional ELISA. Biotinylated rmWnt-5a immobilized on astreptavidincoated plate at 1 μg/mL can bind rhFrizzled-10/ Fc Chimera withan apparent Kd < 35 nM.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• SDS-PAGE: 56-58 kDa, reducingconditions
• Purity: >90%, by SDS-PAGEunder reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 100μg/mL in sterile PBS.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to-70 °C under sterile conditions after reconstitution.

Gene Information

• Gene Name: FZD10 frizzled family receptor10 [ Homo sapiens ]
• Official Symbol: FZD10
• Synonyms: FZD10;frizzled family receptor 10; frizzled (Drosophila) homolog 10; frizzled 10,seven transmembrane spanning receptor; frizzled homolog 10 (Drosophila);frizzled-10; CD350; CD 350; CD 350 antigen; FZ10; FZD 10; FzE7; hFz10;frizzled homolog 10; frizzled 10, seven transmembrane spanning receptor;Fz10; FZ-10; OTTHUMP00000238659
• Gene ID: 11211
• mRNA Refseq: NM_007197
• Protein Refseq: NP_009128
• MIM: 606147
• UniProt ID: Q9ULW2
• Chromosome Location: 12q24.33
• Pathway: Basal cellcarcinoma; Class B/2 (Secretin family receptors); GPCR ligand binding; HTLV-Iinfection; Melanogenesis; Pathways in cancer; Signal Transduction; Signalingby GPCR; Wnt Signaling Pathway; Wnt signaling network
• Function: G-protein coupledreceptor activity; PDZ domain binding; Wnt-activated receptor activity;Wnt-protein binding; protein binding