Active Recombinant Human FURIN protein, His-tagged

• Cat.No.: FURIN-1468H
• Product Overview: Recombinant Human FURIN protein(Gln27-Ala574), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 27-574 a.a.
• Form: Supplied as 0.2 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH9.0 with glycerol as protectant.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate pERTKR-AMC. The specific activity is >125 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 62.1 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln27-Ala574
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FURIN furin (paired basic amino acid cleaving enzyme) [ Homo sapiens ]
• Official Symbol: FURIN
• Synonyms: FURIN; furin (paired basic amino acid cleaving enzyme); FUR, PACE, paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein) , PCSK3; furin; SPC1; FES upstream region; dibasic processing enzyme; dibasic-processing enzyme; furin, membrane associated receptor protein; proprotein convertase subtilisin/kexin type 3; paired basic amino acid residue-cleaving enzyme; FUR; PACE; PCSK3
• Gene ID: 5045
• mRNA Refseq: NM_002569
• Protein Refseq: NP_002560
• MIM: 136950
• UniProt ID: P09958