Active Recombinant Human FZD7 protein, His-tagged

• Cat.No.: FZD7-643H
• Product Overview: Recombinant Human FZD7 protein(Gln 33 - Leu 185), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gln33-Leu185
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Loaded Biotinylated Human Glypican 3, His,Avitag, premium grade on SA Biosensor, can bind Human Frizzled-7, His Tag with an affinity constant of 1.18 μM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 18.6 kDa. The protein migrates as 25-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FZD7 frizzled family receptor 7 [ Homo sapiens ]
• Official Symbol: FZD7
• Synonyms: FZD7; frizzled family receptor 7; frizzled (Drosophila) homolog 7 , frizzled 7, seven transmembrane spanning receptor , frizzled homolog 7 (Drosophila); frizzled-7; FzE3; fz-7; hFz7; frizzled homolog 7; Frizzled, drosophila, homolog of, 7; frizzled 7, seven transmembrane spanning receptor
• Gene ID: 8324
• mRNA Refseq: NM_003507
• Protein Refseq: NP_003498
• MIM: 603410
• UniProt ID: O75084