Active Recombinant Human GCNT2 protein, His-tagged

• Cat.No.: GCNT2-01H
• Product Overview: Recombinant Human GCNT2 protein, fused to His-tag at C-terminus, was expressed in CHO.

Specification

• Description: This gene encodes the enzyme responsible for formation of the blood group I antigen. The i and I antigens are distinguished by linear and branched poly-N-acetyllactosaminoglycans, respectively. The encoded protein is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding different isoforms have been described.
• Source: CHO
• Species: Human
• Tag: His
• Form: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to transfer GlcNAc from UDP-GlcNAc to Cy5-labeled Extended G2.
  0.25 μg of Recombinant Human GCNT2 will convert >50% of its substrate to product, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 43.8 kDa
  SDS-PAGE: 53-67 kDa, reducing conditions
• N-terminal Sequence Analysis: Asn26
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: > 95 %
• Applications: Enzyme Activity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: GCNT2 glucosaminyl (N-acetyl) transferase 2 (I blood group) [ Homo sapiens (human) ]
• Official Symbol: GCNT2
• Synonyms: CCAT; CTRCT13; EC 2.4.1.150; GCNT2; GCNT2C; GCNT5; I beta-1,6-N-acetylglucosaminyltransferase; I-branching beta-1; 6-acetylglucosaminyltransferase; I-branching enzyme; IGNTglucosaminyl (N-acetyl) transferase 5; II; Ii blood group; MGC163396; N-acetylglucosaminyltransferase; N-acetyllactosaminide beta-1; 6-N-acetylglucosaminyl-transferase; N-acetyllactosaminide beta-1; 6-N-acetylglucosaminyltransferase; NACGT1; NAGCT1; ULG3; bA360O19.2; bA421M1.1; beta-1; 6-N-acetylglucosaminyltransferase 2; cataract; congenital; glucosaminyl (N-acetyl) transferase 2; I-branching enzyme, glucosaminyl (N-acetyl) transferase 2; I-branching enzyme (I blood group); glucosaminyl (N-acetyl) transferase 2; I-branching enzyme (Ii blood group)
• Gene ID: 2651
• mRNA Refseq: NM_001491
• Protein Refseq: NP_001482
• MIM: 600429
• UniProt ID: Q8N0V5