Active Recombinant Human GCSF

• Cat.No.: GCSF-89H
• Product Overview: Recombinant Human GCSF produced in CHO cells is a single, glycosylated, polypeptide chain containing 174 amino acids and having a molecular mass of approximately 18 kDa. G-CSF is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: CHO
• Tag: Non
• Description: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder. G-CSF was lyophilized from a concentrated (1mg/ml) Phosphate- Buffered Saline, pH 7.4.
• Bio-activity: The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.07 ng/ml, corresponding to a Specific Activity of 1.27 x 10^8 IU/mg.
• Molecular Mass: 18 kDa
• AA Sequence: TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP.
• Purity: Greater than 97.0% as determined by RP-HPLC and SDS-PAGE.
• Stability: Lyophilized Granulocyte Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution G-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized Granulocyte Colony Stimulating Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: CSF3 colony stimulating factor 3 (granulocyte) [ Homo sapiens (human) ]
• Official Symbol: CSF3
• Synonyms: CSF3; GCSF; CSF3OS; C17orf33; colony stimulating factor 3 (granulocyte); granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin
• Gene ID: 1440
• mRNA Refseq: NM_000759
• Protein Refseq: NP_000750
• MIM: 138970
• UniProt ID: P09919
• Chromosome Location: 17q11.2-q12
• Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Jak-STAT signaling pathway
• Function: cytokine activity; enzyme binding; granulocyte colony-stimulating factor receptor binding