Active Recombinant Human GLO1 protein, His-tagged

• Cat.No.: GLO1-27H
• Product Overview: Recombinant human GLO1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Lactoylglutathione lyase, also known as GLO1, belongs to the glyoxalaseIfamily. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione.This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol
• Bio-activity: Specific activity: >400 units/mg. One unit will form 1.0umol of S-lactoylgutathione from methylglyoxal and reduced glutathioneper minute at pH6.5 at 25C.
• Molecular Mass: 20.7 kDa (184 aa), confirmed by MALDI-TOF
• AA Sequence: ARPCIPKSFG YSSVVCVCNA TYCDSFDPPT FPALGTFSRY ESTRSGRRME LSMGPIQANH TGTGLLLTLQ PEQKFQKVKG FGGAMTDAAALNILALSPPA QNLLLKSYFS EEGIGYNIIR VPMASCDFSI RTYTYADTPD DFQLHNFSLP EEDTKLKIPL IHRALQLAQR PVSLLASPWTSPTWLKTNGA VNGKGSLKGQ PGDIYHQTWA RYFVKFLDAY AEHKLQFWAV TAENEPSAGL LSGYPFQCLG FTPEHQRDFI ARDLGPTLANSTHHNVRLLM LDDQRLLLPH WAKVVLTDPE AAKYVHGIAV HWYLDFLAPA KATLGETHRL FPNTMLFASE ACVGSKFWEQ SVRLGSWDRGMQYSHSIITN LLYHVVGWTD WNLALNPEGG PNWVRNFVDS PIIVDITKDT FYKQPMFYHL GHFSKFIPEG SQRVGLVASQ KNDLDAVALMHPDGSAVVVV LNRSSKDVPL TIKDPAVGFL ETISPGYSIH TYLWRRQHHH HHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GLO1 glyoxalase I [ Homo sapiens ]
• Official Symbol: GLO1
• Synonyms: GLO1; glyoxalase I; lactoylglutathione lyase; GLOD1; glyoxalase domain containing 1; glx I; aldoketomutase; methylglyoxalase; ketone-aldehyde mutase; lactoyl glutathione lyase; S-D-lactoylglutathione methylglyoxal lyase; GLYI
• Gene ID: 2739
• mRNA Refseq: NM_006708
• Protein Refseq: NP_006699
• MIM: 138750
• UniProt ID: Q04760
• Chromosome Location: 6p21.3-p21.1
• Pathway: Pyruvate metabolism, organism-specific biosystem; Pyruvate metabolism, conserved biosystem; methylglyoxal degradation I, organism-specific biosystem
• Function: lactoylglutathione lyase activity; lyase activity; metal ion binding

Reviews

06/28/2022

Timely protein expression optimization; improves our research efficiency.

07/20/2020

Accurate protein structural analysis; crucial for our studies.

11/05/2019

Dependable antibody validation; boosts our immunoprecipitation studies.

Q&As

What role does GLO1 play in the pathogenesis of diabetes and related metabolic disorders?

GLO1 is implicated in diabetes and metabolic disorders, influencing glycation end products and insulin signaling.

What are the mechanisms by which GLO1 modulates cancer cell proliferation and metastasis?

GLO1 modulates cancer cell growth and spread, affecting tumor development and metastasis.

How does GLO1 interact with other components of the glyoxalase system in maintaining cellular homeostasis?

GLO1 works with the glyoxalase system to regulate cellular detoxification and protect against metabolic stress.

How does GLO1 expression influence cellular oxidative stress and aging?

GLO1 expression affects cellular oxidative stress, playing a role in aging and age-related cellular damage.

How do genetic variations in the GLO1 gene affect its enzymatic activity and the susceptibility to chronic diseases?

Genetic variations in GLO1 alter its activity, affecting disease risk and response to metabolic stress.

What is the impact of GLO1 overexpression or deficiency on neurological functions and neurodegenerative diseases?

Changes in GLO1 levels impact neurological function and may contribute to the progression of neurodegenerative diseases.

How does GLO1 regulate the detoxification of methylglyoxal and other reactive aldehydes in cells?

GLO1 detoxifies methylglyoxal and other aldehydes, preventing cellular damage and maintaining metabolic balance.