Active Recombinant Human GPC3 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: GPC3-620HAF555
• Product Overview: The Alexa Fluor 555 conjugated recombinant human GPC3 extracellular or ecto-domain (ECD) is expressed as a 762 amino acid protein consisting of Gln25-Val558 region of GPC3 (Uniprot accession# P51654-3) and a C-terminal Fc fusion from human IgG1, which exists as multiple proteolytically processed species under reducing conditions.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 25-558 a.a.
• Bio-activity: Binds human FGF-basic/FGF2 in a functional ELISA.
• Molecular Mass: Calculated molecular mass (kDa): 86.2; Estimated by SDS-PAGE under reducing condition (kDa): 3 major bands at 40, 60 and 95-125 kDa.
• Endotoxin: < 0.1 EU/ μg of purified recombinant protein determined by the LAL method
• Purity: > 90 % by SDS-PAGE under reducing condition
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: The product is shipped at 4 centigrade. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20 centigrade for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4 centigrade for no more than 2 weeks. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied at 0.5 mg/ml in sterile PBS pH 7.4 (carrier and preservative free).
• Conjugation: Alexa Fluor 555

Gene Information

• Gene Name: GPC3 glypican 3 [ Homo sapiens ]
• Official Symbol: GPC3
• Synonyms: GPC3; glypican 3; SDYS; glypican-3; DGSX; glypican proteoglycan 3; OCI 5; SGB; SGBS; SGBS1; secreted glypican-3; intestinal protein OCI-5; heparan sulphate proteoglycan; MXR7; OCI-5; GTR2-2
• Gene ID: 2719
• mRNA Refseq: NM_001164617
• Protein Refseq: NP_001158089
• MIM: 300037
• UniProt ID: P51654