Active Recombinant Human GPC3 protein, His-tagged, Low Endotoxin

• Cat.No.: GPC3-2551H
• Product Overview: Recombinant Human GPC3 protein(P51654-1)(Gln25-His559), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 25-559 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human GPC3, His Tag at 1 μg/ml (100μl/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 61.6 kDa. Due to furin-like convertase cleavage site and glycosylation, the protein migrates to 30-31 kDa, 38-42 kDa and 65-80 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: GPC3 glypican 3 [ Homo sapiens ]
• Official Symbol: GPC3
• Synonyms: GPC3; glypican 3; SDYS; glypican-3; DGSX; glypican proteoglycan 3; OCI 5; SGB; SGBS; SGBS1; secreted glypican-3; intestinal protein OCI-5; heparan sulphate proteoglycan; MXR7; OCI-5; GTR2-2
• Gene ID: 2719
• mRNA Refseq: NM_001164617
• Protein Refseq: NP_001158089
• MIM: 300037
• UniProt ID: P51654