Active Recombinant Human HDAC5 Protein

• Cat.No.: HDAC5-312H
• Product Overview: Recombinant Human HDAC5 Protein is produced by HEK293 cells expression system.

Specification

• Description: Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.
• Source: HEK293 cells
• Species: Human
• Form: Liquid, In 30 mM HEPES, 140 mM NaCl, 10 mM KCl, 3% glycerol, 0.25 mM TCEP, pH 7.6.
• Bio-activity: Deacetylation activity was determined using the Boc-K(TFA)-AMC substrate. Reactions were carried out in a 384-well plate in 20 µL of reaction buffer comprising of 50 mM HEPES, 140 mM NaCl, 10 mM KCl, 1 mg/ml bovine serum albumin (BSA), and 1 mM TCEP, pH 7.4, for 30 min at 37°C. The reaction was stopped by addition of 20 µl of trypsin solution (2 mg/ml trypsin) and the fluorescence signal of released AMC was quantified using a fluorometer. The enzyme kinetic values were calculated by nonlinear regression analysis.
• Molecular Mass: 45 kDa
• Protein length: amino acids 679-1095
• Purity: ≥95% by SDS-PAGE
• Notes: For Research Use Only! Not For Use in Humans.
• Storage: Store at -80 centigrade.
• Handling: Thaw on ice, and aliquot into smaller working quantities to avoid multiple freeze/thaw cycles.

Gene Information

• Gene Name: HDAC5 histone deacetylase 5 [ Homo sapiens ]
• Official Symbol: HDAC5
• Synonyms: HDAC5; histone deacetylase 5; FLJ90614; KIAA0600; NY CO 9; antigen NY-CO-9; HD5; NY-CO-9
• Gene ID: 10014
• mRNA Refseq: NM_001015053
• Protein Refseq: NP_001015053
• MIM: 605315
• UniProt ID: Q9UQL6