| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Non |
| Protein Length : |
amino acids 679-1095 |
| Description : |
Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions. |
| Form : |
Liquid, In 30 mM HEPES, 140 mM NaCl, 10 mM KCl, 3% glycerol, 0.25 mM TCEP, pH 7.6. |
| Bio-activity : |
Deacetylation activity was determined using the Boc-K(TFA)-AMC substrate. Reactions were carried out in a 384-well plate in 20 µL of reaction buffer comprising of 50 mM HEPES, 140 mM NaCl, 10 mM KCl, 1 mg/ml bovine serum albumin (BSA), and 1 mM TCEP, pH 7.4, for 30 min at 37°C. The reaction was stopped by addition of 20 µl of trypsin solution (2 mg/ml trypsin) and the fluorescence signal of released AMC was quantified using a fluorometer. The enzyme kinetic values were calculated by nonlinear regression analysis. |
| Molecular Mass : |
45 kDa |
| Purity : |
≥95% by SDS-PAGE |
| Notes : |
For Research Use Only! Not For Use in Humans. |
| Storage : |
Store at -80 centigrade. |
| Handling : |
Thaw on ice, and aliquot into smaller working quantities to avoid multiple freeze/thaw cycles. |
