Active Recombinant Human Histone Deacetylase 1, His-tagged

• Cat.No.: HDAC1-392H
• Product Overview: HDAC1 was cloned from human brain cDNA and expressed in insect cells using a baculoviral expression system.Full Length Human HDAC1 – Class I, 59 kD with his tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
• Purity: Purified, active enzyme.
• Purification Procedure: Ni2+ Affinity Column
• Activity: 1000 units per vial. 1 unit is defined as the amount of enzyme that will convert 1picomole per minute per ug at pH8 at 30°C.
• Stability/Shelf-life: >6 months from date of shipment
• Buffer Composition: 25mM Tris pH8, 120mM NaCl, 0.05% Tween-20 & 40% Glycerol
• Storage: –80°C
• Full Length: Full L.
• Publications:
  Circumvention of Gefitinib Resistance by Repurposing Flunarizine via Histone Deacetylase Inhibition (2023)

Gene Information

• Gene Name: HDAC1 histone deacetylase 1 [ Homo sapiens ]
• Synonyms: HDAC1; DKFZp686H12203; GON-10; HD1; RPD3; RPD3L1; histone deacetylase 1; OTTHUMP00000008745; reduced potassium dependency, yeast homolog-like 1; DKFZp686H12203; EC 3.5.1.98.
• Gene ID: 3065
• mRNA Refseq: NM_004964
• Protein Refseq: NP_004955
• MIM: 601241
• UniProt ID: Q13547
• Chromosome Location: 1p34
• Pathway: Cell cycle; Chronic myeloid leukemia; Notch signaling pathway; Gene Expression; Signalling by NGF
• Function: enzyme binding; histone deacetylase activity; hydrolase activity; identical protein binding; transcription activator activity; transcription factor activity; transcription factor binding; transcription factor binding