Active Recombinant Human ICAM3, Fc Chimera

• Cat.No.: ICAM3-493H
• Product Overview: Recombinant Human ICAM3 (Accession # CAA49473) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Predicted N Terminal: No results obtained: Gln30 predicted
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.When 5 x 10^4 cells/well are added to rhICAM-3/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), approximately 40 - 70% will adhere after PMA stimulation for 1 hour at room temperature.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human ICAM3, Fc Chimera has a calculated MW of 76.7 kDa (monomer). In SDS-PAGE migrates as 120 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ICAM3 intercellular adhesion molecule 3 [ Homo sapiens ]
• Official Symbol: ICAM3
• Synonyms: ICAM3; intercellular adhesion molecule 3; CD50; CDW50; ICAM R; ICAM-3; intercellular adhesion molecule-3; ICAM-R
• Gene ID: 3385
• mRNA Refseq: NM_002162
• Protein Refseq: NP_002153
• MIM: 146631
• UniProt ID: P32942
• Chromosome Location: 19p13.3-p13.2