Active Recombinant Human IGF1 protein, Fc-tagged

• Cat.No.: IGF1-0246H
• Product Overview: Recombinant Human IGF1 protein(Gly49-Ala118), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human IGF-I, Fc Tag at 5 μg/mL (100 μL/well) can bind Human IGFBP-3, His Tag with a linear range of 0.125-1 μg/mL.Immobilized Human IGF-I R, His Tag at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag with a linear range of 0.039-0.313 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the N-terminus.The protein has a calculated MW of 34.6 kDa. The protein migrates as 40 kDa under reducing (R) condition (SDS-PAGE).
• Proteinlength: Gly49-Ala118
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >98% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IGF1 insulin-like growth factor 1 (somatomedin C) [ Homo sapiens ]
• Official Symbol: IGF1
• Synonyms: IGF1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; IGF1A; MGF; IGF-IA; IGF-IB; somatomedin-C; mechano growth factor; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; IGFI; IGF-I
• Gene ID: 3479
• mRNA Refseq: NM_000618
• Protein Refseq: NP_000609
• MIM: 147440
• UniProt ID: P05019