Active Recombinant Human IGF1R protein, His-tagged

• Cat.No.: IGF1R-180H
• Product Overview: Active Recombinant Human IGF1R protein(P08069)(Glu31-Asn932) is expressed from HEK293 with His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 31-932 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human IGF1R, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 0.90μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 80.4 kDa (alpha subunit) and 24.1 kDa (beta subunit). Due to the furin cleavage site and glycosylation, the protein migrates to 110-130 kDa and 45-55 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: IGF1R insulin-like growth factor 1 receptor [ Homo sapiens ]
• Official Symbol: IGF1R
• Synonyms: IGF1R; insulin-like growth factor 1 receptor; CD221; IGFIR; IGFR; JTK13; MGC18216; IGF-I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor I receptor; MGC142170; MGC142172
• Gene ID: 3480
• mRNA Refseq: NM_000875
• Protein Refseq: NP_000866
• MIM: 147370
• UniProt ID: P08069