Active Recombinant Human IL11RA protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: IL11RA-0196H
• Product Overview: Biotinylated Recombinant Human IL11RA protein(Ser24-Ala370), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc&Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human IL-11, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-11 R alpha, Fc,Avitag with a linear range of 2-39 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 66.0 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ser24-Ala370
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL11RA interleukin 11 receptor, alpha [ Homo sapiens ]
• Official Symbol: IL11RA
• Synonyms: IL11RA; interleukin 11 receptor, alpha; interleukin-11 receptor subunit alpha; IL-11RA; IL-11R subunit alpha; IL-11 receptor subunit alpha; interleukin-11 receptor alpha chain; CRSDA; MGC2146
• Gene ID: 3590
• mRNA Refseq: NM_001142784
• Protein Refseq: NP_001136256
• MIM: 600939
• UniProt ID: Q14626

Reviews

02/15/2021

High affinity binding.

09/05/2020

Stable in solution.

02/20/2017

Effective in hematopoiesis studies.

Q&As

Can you explain how phosphoproteomics and mass spectrometry shed light on IL11RA-mediated signaling cascades and post-translational modifications?

Phosphoproteomics and mass spectrometry reveal IL11RA-related signaling pathways.

How are flow cytometry and fluorescence-activated cell sorting (FACS) employed to analyze IL11RA expression on different cell populations?

Flow cytometry and FACS analyze IL11RA expression on different cell types.

Could you elaborate on how X-ray crystallography and cryo-electron microscopy contribute to understanding the three-dimensional structure of IL11RA?

X-ray crystallography and cryo-EM reveal the three-dimensional structure of IL11RA.

What insights have gene expression profiling and RNA sequencing provided regarding IL11RA-related molecular pathways and networks?

Gene expression profiling and RNA sequencing unveil IL11RA-associated molecular networks.

How do techniques like co-immunoprecipitation and pull-down assays validate IL11RA's interactions with IL11 and other signaling partners?

Co-immunoprecipitation and pull-down assays confirm IL11RA interactions with IL11 and other partners.

What role does site-directed mutagenesis play in identifying IL11RA residues critical for IL11 binding and downstream signaling activation?

Site-directed mutagenesis identifies IL11RA residues crucial for binding and signaling.

How are animal models like transgenic mice and tissue-specific knockouts utilized to study the in vivo roles of IL11RA in development and disease?

Transgenic mice and knockouts study IL11RA's roles in development and disease.

Can you explain the experimental strategies used to characterize IL11RA isoforms and their differential roles in IL11 signaling?

Experimental strategies characterize IL11RA isoforms and their distinct roles in IL11 signaling.

How are biophysical techniques such as isothermal titration calorimetry and surface plasmon resonance used to determine IL11RA's binding affinities and kinetics?

Isothermal titration calorimetry and surface plasmon resonance measure IL11RA binding properties.

What methods like CRISPR-Cas9 knockout and RNA interference help elucidate the functional significance of IL11RA in specific biological contexts?

CRISPR-Cas9 knockout and RNA interference reveal IL11RA's functions in specific contexts.