Recombinant Mouse IL11RA cell lysate

Cat.No. : IL11RA-2558MCL
Product Overview : Mouse IL11RA / IL11Rα derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Il11ra1 interleukin 11 receptor, alpha chain 1 [ Mus musculus ]
Official Symbol : Il11ra1
Synonyms : NR1; GP130; Il11ra; Il-11ra; Il11ra2; AI314697; interleukin-11 receptor subunit alpha-1; NR-1; etl-2; locus 2; IL-11RA1; Il-11ra-alpha; IL-11R-alpha-1; IL-11R subunit alpha-1; novel cytokine receptor 1; enhancer trap locus homolog 2; IL-11 receptor subunit alpha-1; interleukin 11 receptor, alpha chain 2
Gene ID : 16157
mRNA Refseq : NM_001163401
Protein Refseq : NP_001156873
UniProt ID : Q64385
Chromosome Location : 4 A5; 4 21.94 cM
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem
Function : cytokine receptor activity; interleukin-11 binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Can you explain how phosphoproteomics and mass spectrometry shed light on IL11RA-mediated signaling cascades and post-translational modifications? 04/16/2022

Phosphoproteomics and mass spectrometry reveal IL11RA-related signaling pathways.

How are flow cytometry and fluorescence-activated cell sorting (FACS) employed to analyze IL11RA expression on different cell populations? 07/13/2021

Flow cytometry and FACS analyze IL11RA expression on different cell types.

Could you elaborate on how X-ray crystallography and cryo-electron microscopy contribute to understanding the three-dimensional structure of IL11RA? 06/12/2020

X-ray crystallography and cryo-EM reveal the three-dimensional structure of IL11RA.

What insights have gene expression profiling and RNA sequencing provided regarding IL11RA-related molecular pathways and networks? 09/10/2019

Gene expression profiling and RNA sequencing unveil IL11RA-associated molecular networks.

How do techniques like co-immunoprecipitation and pull-down assays validate IL11RA's interactions with IL11 and other signaling partners? 08/29/2019

Co-immunoprecipitation and pull-down assays confirm IL11RA interactions with IL11 and other partners.

What role does site-directed mutagenesis play in identifying IL11RA residues critical for IL11 binding and downstream signaling activation? 07/28/2019

Site-directed mutagenesis identifies IL11RA residues crucial for binding and signaling.

How are animal models like transgenic mice and tissue-specific knockouts utilized to study the in vivo roles of IL11RA in development and disease? 05/18/2019

Transgenic mice and knockouts study IL11RA's roles in development and disease.

Can you explain the experimental strategies used to characterize IL11RA isoforms and their differential roles in IL11 signaling? 04/08/2018

Experimental strategies characterize IL11RA isoforms and their distinct roles in IL11 signaling.

How are biophysical techniques such as isothermal titration calorimetry and surface plasmon resonance used to determine IL11RA's binding affinities and kinetics? 03/08/2018

Isothermal titration calorimetry and surface plasmon resonance measure IL11RA binding properties.

What methods like CRISPR-Cas9 knockout and RNA interference help elucidate the functional significance of IL11RA in specific biological contexts? 10/18/2017

CRISPR-Cas9 knockout and RNA interference reveal IL11RA's functions in specific contexts.

Customer Reviews (3)

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Reviews
02/15/2021

    High affinity binding.

    09/05/2020

      Stable in solution.

      02/20/2017

        Effective in hematopoiesis studies.

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