Recombinant Canine IL11RA cell lysate

• Cat.No.: IL11RA-001CCL
• Product Overview: Canine IL11RA / IL-11RA / IL11Rα derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.

Specification

• Source: Human cells
• Species: Canine
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: IL11RA interleukin 11 receptor, alpha [ Canis lupus familiaris ]
• Official Symbol: IL11RA
• Synonyms: interleukin-11 receptor subunit alpha; interleukin 11 receptor, alpha
• Gene ID: 481588
• mRNA Refseq: XM_005626742
• Protein Refseq: XP_005626799
• Chromosome Location: chromosome: 11
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem

Reviews

02/15/2021

High affinity binding.

09/05/2020

Stable in solution.

02/20/2017

Effective in hematopoiesis studies.

Q&As

Can you explain how phosphoproteomics and mass spectrometry shed light on IL11RA-mediated signaling cascades and post-translational modifications?

Phosphoproteomics and mass spectrometry reveal IL11RA-related signaling pathways.

How are flow cytometry and fluorescence-activated cell sorting (FACS) employed to analyze IL11RA expression on different cell populations?

Flow cytometry and FACS analyze IL11RA expression on different cell types.

Could you elaborate on how X-ray crystallography and cryo-electron microscopy contribute to understanding the three-dimensional structure of IL11RA?

X-ray crystallography and cryo-EM reveal the three-dimensional structure of IL11RA.

What insights have gene expression profiling and RNA sequencing provided regarding IL11RA-related molecular pathways and networks?

Gene expression profiling and RNA sequencing unveil IL11RA-associated molecular networks.

How do techniques like co-immunoprecipitation and pull-down assays validate IL11RA's interactions with IL11 and other signaling partners?

Co-immunoprecipitation and pull-down assays confirm IL11RA interactions with IL11 and other partners.

What role does site-directed mutagenesis play in identifying IL11RA residues critical for IL11 binding and downstream signaling activation?

Site-directed mutagenesis identifies IL11RA residues crucial for binding and signaling.

How are animal models like transgenic mice and tissue-specific knockouts utilized to study the in vivo roles of IL11RA in development and disease?

Transgenic mice and knockouts study IL11RA's roles in development and disease.

Can you explain the experimental strategies used to characterize IL11RA isoforms and their differential roles in IL11 signaling?

Experimental strategies characterize IL11RA isoforms and their distinct roles in IL11 signaling.

How are biophysical techniques such as isothermal titration calorimetry and surface plasmon resonance used to determine IL11RA's binding affinities and kinetics?

Isothermal titration calorimetry and surface plasmon resonance measure IL11RA binding properties.

What methods like CRISPR-Cas9 knockout and RNA interference help elucidate the functional significance of IL11RA in specific biological contexts?

CRISPR-Cas9 knockout and RNA interference reveal IL11RA's functions in specific contexts.